Journal: Nature immunology

Article Title: Subsets of ILC3-ILC1-like cells generate a diversity spectrum of ILCs in human mucosal tissues.

doi: 10.1038/s41590-019-0425-y

Figure Lengend Snippet: a, The viSNE analysis of tonsil NKp44+ cells based on 36 selected markers and colored by intensity of expression. Each plot shows expression of the indicated cell surface marker. Populations corresponding to ILC3a, ILC3b, ILC1b and ILC1a are delineated in the CD127 plot. Cell subsets that lack CD103, but express CD94, NKG2A and CD127 are indicated as “Others”. b, Heat maps show graded expression of the indicated markers in the ILC subsets. Two donors analyzed in parallel on the same day are shown for consistency. c, The viSNE map of NKp44+CD103+ cells shows 3 clusters in which the degree of expression of CD127 and CCR6 inversely correlates with that of CD94. The viSNE analysis also identifies a small ILC1 cluster expressing CD16. Data are representative of six donors. Cells were gated based on NKp44 and CD161 (a,b) or NKp44, CD161 and CD103 (c) before running viSNE. Contaminating CD3+CD19+FceR+TREM1+CD14+ cells were excluded by manual gating.

Article Snippet: All the following antibodies were purchased from Fluidigm and included in the panel CD45–089Y (3089003B); CD196–141Pr (3141003A); CD19–142Nd (3142001B); CD117–143Nd (3143001B); CD4–145Nd (3145001B); CD8a-146Nd (3146001B); CD25–149Sm (3149010B); FceR-150Nd (3150027B); CD138–150Nd (3150012B); CD103–151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3–154Sm (3154003B); CD85j-156Gd (3156020B); CD194–158Gd (3158032A); CD161–159Tb (3159004B); CD39–160Gd (3160004B); CD27–162Dy (3162009B); CD45RO-165Ho (3165011B); CD34–166Er (3166012B); CD127–168Er (3168017B); CD159–169Tm (3169013B); CD45RA-170Er(3170010B); CD226–171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94–174Yb (3174015B); CD14–175Lu (3175015B); CD56–176Yb (3176009B) and CD16–299Bi (3209002B).

Techniques: Expressing, Marker

Journal: Scientific Reports

Article Title: Identification of Genes Associated with Smad3-dependent Renal Injury by RNA-seq-based Transcriptome Analysis

doi: 10.1038/srep17901

Figure Lengend Snippet: Validation of RNA-sequencing results by real-time PCR.

Article Snippet: I (Southernbiotech, USA), Trem1 and Pvalb (boster,Wuhan, China), β-actin(Santa Cruz, USA), followed by horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature.

Techniques: Biomarker Discovery

Journal: Annals of Medicine

Article Title: TREM-1, TREM-2 and their association with disease severity in patients with COVID-19

doi: 10.1080/07853890.2023.2269558

Figure Lengend Snippet: Expression of TREM-1 and TREM-2 on the surface of cell subpopulations. Flow cytometric analysis of TREM-1 expression on the surface of monocytes (A), CD4 + T cells (C), CD8 + T cells (E) and B cells (G) from non-pneumonia donors ( n = 50) and patients with moderate ( n = 34) and severe COVID-19 ( n = 44). Flow cytometric analysis of TREM-2 expression on the surfaces of monocytes (B), CD4 + T cells (D), CD8 + T cells (F) and B cells (H) from non-pneumonia donors or patients with COVID-19. Data are displayed as the mean ± SD. Statistical significance was determined by an unpaired t -test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: WBCs were thawed and analysed using a panel of antibodies as follows: FITC anti-human CD3 (clone UCHT1, TONBO), APC-Cyanine7 anti-human CD4 (clone RPA-T4, TONBO), PerCP-Cyanine5.5 anti-human CD8a (clone OKT8, TONBO), PE/DazzleTM 594 anti-human CD19 (clone HIB19, BioLegend), PE-Cyanine7 anti-human CD14 (clone 61D3, TONBO), PE anti-human CD354 (TREM-1, BioLegend) and human/mouse TREM2 APC-conjugated antibody (clone 237920, R&D Systems).

Techniques: Expressing